Can corona tests be used to collect bacteria from the mouth and throat for experiments?
We have to design an experiment for MINT (10th grade high school) to test the effects of essential oils. Our idea was to use coronavirus tests, or rather their sterile cotton swabs, as a sampling medium so that we can inoculate the culture medium with bacteria from our mouths and throats. Now the question is what this would look like in practice. Can we use the liquid included with the tests to squeeze the swabs, as in the actual testing procedure? Or do we need to get other materials for that?
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The solution of the coronatests serves the lysis of the cells contained in the sample in order to release virus proteins which are located in the mucous membrane cells. You’d like to inoculate living cells on a culture plate. If you were to put these in the lysis buffer, the bacteria cells would also be broken. So you can take the Tupfer theoretically, I’d guess about the buffer.
You can take the sticks and do it and do it again. There should be a simple leap if you don’t just try to brush your teeth
If the tupfers are not specifically treated in order to achieve preselectione, all germs can be removed in principle.
Pressing out is not absolutely necessary. The bacteria on the surface should be completely sufficient for cultivation. If you still want to use the liquid please make sure that the volume which is brought to a standard 9 cm plate should not exceed 1 ml.
In addition, you would have to distribute the liquid by means of spatula processes.